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Specimens was basically checked out to your exposure off HPV of the amplifying 50 µ l of your DNA extracts with the Linear Assortment HPV genotyping sample adopting the instructions on brand (Roche Diagnostics, Indianapolis, IN). Trials was increased playing with Perkin-Elmer GeneAmp PCR Program 9700 as directed by linear number process. HPV genotyping try presented towards the the samples regardless of HPV PCR result. fourteen ? -globin is thought of within the 99.7% off examples examined (). Trials that increased HPV for the PCR but didn’t hybridize with a particular HPV sort of with...
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